Journal: Stem Cells International

Article Title: Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients

doi: 10.1155/2019/7532657

Figure Lengend Snippet: Patch clamp analysis of cardiomyocytes from control- and LQT-iPSC lines. (a) Representative APs of 1 Hz paced a control-iPSC-CM and an LQT-iPSC-CM from II-2 in . Ten consecutive waves are shown. (b) MDP, APA, and APD 90 from cardiomyocytes derived from the six lines: 201B7 ( n = 6), 409B2 ( n = 5), 692D2 ( n = 6), LQT1A1 ( n = 5), LQT1B1 ( n = 5), and LQT1C1 ( n = 7). Data are represented as means ± SEM; ∗∗ p < 0.005. (c) Representative current traces from control- and LQT1-iPSC-CMs. Upper, the protocol in current clamp recording. Middle, representative traces before and after perfusion with 3R4S-chromanol 293B (30 μ mol/l). Lower, 3R4S-chromanol 293B-subtraction. (d) I-V plots of I Ks at the end of the depolarizing step. 692D2 (control) ( n = 3), LQT1B1 ( n = 3); ∗ p < 0.05.

Article Snippet: Binning was set to 4 × 4, and the measurement interval was every 4 ms. Cardiomyocyte monolayers 7–10 days after seeding on each well were stimulated at 1 Hz with 1 ms depolarizing pulses at 10 V. Analysis of APD 90 was performed with FDSS Waveform Analysis software for cardiomyocytes (U8524-12; Hamamatsu Photonics).

Techniques: Patch Clamp, Control, Derivative Assay

Journal: Stem Cells International

Article Title: Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients

doi: 10.1155/2019/7532657

Figure Lengend Snippet: AP measurements of single cells using a membrane potential dye. (a) Examples of AP waves from control (409B2) measured by the dye. ROIs (1)–(5) are ventricular type. ROI (5) (green wave) shows long APD. (b) Examples of AP waves from LQT1B1. ROIs (1)–(4) are ventricular type with long APD. ROI (4) (purple wave) shows EADs. ROI (5) (green wave) is nodal type. (c) Proportion of subtypes in 3 control- and 3 LQT1-iPSC-CMs: 201B7 ( n = 128), 409B2 ( n = 41), 692D2 ( n = 92), LQT1A1 ( n = 192), LQT1B1 ( n = 101), and LQT1C1 ( n = 202). Ventricular-type cells predominate in all six lines. (d) Representative EADs in a ventricular-type cell from LQT1B1. The EADs were defined as over 20% of the AP amplitude. Arrows show EADs. (e) Occurrence ratio of EADs in ventricular cardiomyocytes from the six lines. The number of ventricular cells was used as the denominator; ∗ p < 0.05.

Article Snippet: Binning was set to 4 × 4, and the measurement interval was every 4 ms. Cardiomyocyte monolayers 7–10 days after seeding on each well were stimulated at 1 Hz with 1 ms depolarizing pulses at 10 V. Analysis of APD 90 was performed with FDSS Waveform Analysis software for cardiomyocytes (U8524-12; Hamamatsu Photonics).

Techniques: Membrane, Control

Journal: Stem Cells International

Article Title: Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients

doi: 10.1155/2019/7532657

Figure Lengend Snippet: AP measurements in a cardiomyocyte monolayer. Data are represented as means ± SEM. (a) An example phase contrast image of the monolayer culture. Scale bar, 500 μ m. (b) Representative AP of a paced control-iPSC-CM monolayer. APD 90 was calculated from the average of 10 consecutive waves. (c) APD 90 from 3 control- and 3 LQT-iPSC-CM monolayers: 201B7 ( n = 3), 409B2 ( n = 3), 692D2 ( n = 3), LQT1A1 ( n = 3), LQT1B1 ( n = 3), and LQT1C1 ( n = 3). LQT-iPSC-CMs showed longer APD than controls; ∗∗ p < 0.005.

Article Snippet: Binning was set to 4 × 4, and the measurement interval was every 4 ms. Cardiomyocyte monolayers 7–10 days after seeding on each well were stimulated at 1 Hz with 1 ms depolarizing pulses at 10 V. Analysis of APD 90 was performed with FDSS Waveform Analysis software for cardiomyocytes (U8524-12; Hamamatsu Photonics).

Techniques: Control

Journal: Stem Cells International

Article Title: Optical Recording of Action Potentials in Human Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Generated from Long QT Syndrome Type 1 Patients

doi: 10.1155/2019/7532657

Figure Lengend Snippet: AP measurements in cardiomyocyte monolayers using a high-throughput plate reader. (a) Representative AP waves on a 96-well plate. Pacing at 1 Hz starts 10 s after recording. (b) Cisapride prolonged APD 90 of both control- and LQT1-iPSC-CMs; 692D2 (control) ( n = 3), LQT1B1 ( n = 5). (c) Cisapride prolonged APD 90 of both control- and LQT1-iPSC-CMs with 100 nM isoproterenol; 692D2 ( n = 5), LQT1B1 ( n = 3). (d) Erythromycin prolonged APD 90 of both control- and LQT1-iPSC-CMs with 100 nM isoproterenol; 692D2 ( n = 4), LQT1B1 ( n = 3). LQT-iPSC-CM monolayers in 300 nM and 1000 nM cisapride were not synchronized at 1 Hz pacing. Data are represented as means ± SEM; ∗∗ p < 0.005, ∗ p < 0.05.

Article Snippet: Binning was set to 4 × 4, and the measurement interval was every 4 ms. Cardiomyocyte monolayers 7–10 days after seeding on each well were stimulated at 1 Hz with 1 ms depolarizing pulses at 10 V. Analysis of APD 90 was performed with FDSS Waveform Analysis software for cardiomyocytes (U8524-12; Hamamatsu Photonics).

Techniques: High Throughput Screening Assay, Control

Journal: Blood research

Article Title: Characterization of anti-factor VIII antibody in a patient with acquired hemophilia A

doi: 10.5045/br.2013.48.1.58

Figure Lengend Snippet: Clinical course of our patient. This graph shows FVIII (%), aPTT (s), and FVIII Ab (BU, Bethesda unit) levels. Abbreviations: rFVIIa, recombinant activated factor VII; Pd, prednisolone; Aza, azathioprine; HD, hospital day.

Article Snippet: APTT waveform analysis (MDA series; Organon, Technika) of the patient's plasma showed a delayed clotting pattern compared to normal pooled plasma ( ).

Techniques: Recombinant

Journal: Blood research

Article Title: Characterization of anti-factor VIII antibody in a patient with acquired hemophilia A

doi: 10.5045/br.2013.48.1.58

Figure Lengend Snippet: The APTT waveform analysis of the patient's plasma. Minor hemostatic effect by infusion. Abbreviations: Patient Pre, Pre-treatment; Patient Post, Post-treatment; NP, normal pooled plasma; Coag, coagulation.

Article Snippet: APTT waveform analysis (MDA series; Organon, Technika) of the patient's plasma showed a delayed clotting pattern compared to normal pooled plasma ( ).

Techniques: Clinical Proteomics, Coagulation

Journal: Frontiers in Pharmacology

Article Title: High-Throughput Drug Screening System Based on Human Induced Pluripotent Stem Cell-Derived Atrial Myocytes ∼ A Novel Platform to Detect Cardiac Toxicity for Atrial Arrhythmias

doi: 10.3389/fphar.2021.680618

Figure Lengend Snippet: Cardiac differentiation from hiPSCs (A) Schematic of the protocol for the differentiation of cardiomyocytes from hiPS cells with (RA (+)) or without RA (RA (−)) treatment (B) The ratio of cardiomyocytes quantified by immunostaining with cardiac troponin-T (cTnT). The proportion of cTnT positive cells was 93.91 ± 2.51% in CT (control hiPS-CMs), or 96.79 ± 1.81% in AM (RA-treated hiPS-CMs), respectively (P = N.S.). Error bars represent SD of the mean from the values of independent experiments. n = 3. N.S., not significant (C) Immunofluorescence analysis of the percentage of MLC2a (atrial isoform) and MLC2v (ventricular isoform) positive expression in the CT and AM. Error bars represent SD of the mean from the values of independent experiments. ** p < 0.01 for comparison with MLC 2a+/MLC 2v-cardiomyocyte in CT, ## p < 0.01 for comparison with MLC 2a+/MLC 2v-cardiomyocyte in AM, n = 3.

Article Snippet: Recording data were analyzed using Waveform Analysis of Cardiomyocyte Software (Ver.1.2.1J, Hamamatsu Photonics K.K.).

Techniques: Immunostaining, Control, Immunofluorescence, Expressing, Comparison